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Elena NicolaouEN

Elena Nicolaou

Life Science Research; Team Leader

€355/day
London, GB
8-15 years

Average response time: 1 hour

About Elena

I have over 20 years of experience working in a laboratory as a senior and team leader scientist, and office environment, in academia, NHS, and in industry. My skills range from Interior Fit-out, team management, program management, lab and office design, life sciences and CV writer.

I achieved my BSc in Physiology from the University of London and went on to complete an MSc at VUB Brussels, in the area of molecular biology, and immunotherapy.

Subsequently, I was employed in NHS working in Haematology-Oncology Departments carrying out magnetic cell isolation, DNA/RNA extraction, Stem cell isolation, and growing expansion of Mesenchymal stem cells for transplantation. GMP/GLP experience, PCR, protein expression as well as laboratory and team management. Among my responsibilities, I was responsible for sourcing premises, agreeing on leases and contracts, the fit-out of the site, and bringing it to the standard required for laboratory and office work. Was directly involved in recruitment by screening individuals, conducting interviews, and prepared employment contracts, and provided onboarding induction and training to new starters, conducting intricate analysis of payroll processes

As a skilled scientist, I can support both the operational and scientific needs of a company, as I am able to understand the science and laboratory to function efficiently.
- I can work as a consultant providing client-focused support with lab and office setup and fit-outs.
- Unique ability to understand the science and ensure spaces are setup to maximize lab users’ experience and ensure the work can be carried out as effectively as possible.
  • English

    Native or bilingual

  • Greek

    Native or bilingual

  • German

    Conversational

Remote only
Primarily works remotely

Experience

  • UCLH
    Bank Staff
    May 2022 - Today (4 years and 1 month)
    London, UK
  • Cignpost Diagnostics Ltd
    Senior Scientist
    September 2020 - May 2022 (1 year and 8 months)
  • NHS
    BMS
    November 2018 - June 2020 (1 year and 7 months)
    London, UK
    STEM CELL THERAPY-HAEMATOLOGY/ONCOLOGY Performed all aspects of leukemia-lymphoma immunophenotyping by BD CANTO II and DIVA and analyzed data; Quantification and analysis of CD34+ / CD3+ cells. Autologous and Allogeneic Stem Cell Transplantation Programme; Calculations, results and reports prepared; issued of results
    • Use of Trucount tubes;
    • Multipotent Mesenchymal Stromal Cells (MSC) Phenotyping; cultured MSCs stained with the MSC Phenotyping Cocktail and flowed; Isotype controls used
    • Fluorochromes for flow cytometry: FITC, PE, APC, PercP
    • Isolated WBC from Peripheral Blood; 4/6 colour cell surface and intracellular staining; Interferon Gamma Release Assays (IGRAs) for viruses like EBV, HPV, CMV, BKV, COVID-19; No live viruses used; Peptivators kits from Myltenyi Biotec used and Unstained controls;
    • Worked closely with the Head of Quality to ensure regulatory requirements (HTA/ JACIE/ MHRA) are met in all areas of stem cell laboratory work; Maintain appropriate records and documentation. Production, maintenance, implementation of standard operating procedures, continuous improvement of the Quality Management System; Take part into the External Quality Assessment scheme from NEQAs; working in an environment covered by the requirements of GMP and GCLP (Good Control Laboratory Practice)/Asceptic techniques; change controls
    • Undertake quality control procedures, examined quality control results to identify poor performance; instigated appropriate action. Delegated responsibility for the quality control, operation, routine maintenance, troubleshooting of equipment. Prepared environmental monitoring of clean rooms reports and analysed by excel, COSHH the same all Service Legal Agreement (SLAs), Standard Operating Procedure (SOP) Reviewed and update; data entry on stemsoft, updated of laboratory databases for MSC, DLI, CTLs
    • Use of Cerner and Q-Pulse software to retrieve Standard Operational Procedure documents and forms
    - Clinical trials: CRISP, OPUS

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Education

  • Master of Science
    Vrije University Brussels
    2005
    Master in Science, Molecular Biology and Biotechnology
  • Bachelor of Science in Physiology
    KING'S COLLEGE LONDON
    2001
    Bachelor's Degree, Physiology, General

Skill set (5)

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