About Pranav
I was always interested in translational research and I have around 5+ years of experience in the multiple domains of Biotechnology and Lifesciences. I have a strong understanding of molecular biology, biochemistry, cellular biology and neuroscience as well as strong technical skills doing in vivo and in vitro studies using transgenic pre-clinical mouse models, Immunohistochemistry, Immunofluorescence, Scanning electron microscopy, RNAi, CRISPR cas9, molecular biology techniques and a sound knowledge of Imaging.
I have worked on multiple projects through out my experiences thus have a multidisciplinary background good understanding of the available in many domains of Biotechnology.
English
Fluent
Hindi
Native or bilingual
French
Conversational
Can work on-site
Paris (up to 50km)
Experience
- Institut Pasteur ParisPost Doctoral ResearcherRESEARCHJanuary 2020 - January 2022 (2 years)Paris, France• Generated a double mutant pre-clinical model to study the compensation and dependency in hearing and balance disorders of the identical genes.• Initiated, executed and successfully completed multiple research project on time.• Prepare technical reports, papers, and records as well as contributing to the production of research documentation for publication.• Designed and validated guide RNAs using a CRISPR Cas9 machinery in a complex cell line.
- Central Drug Research InstituteResearch AssistantRESEARCHAugust 2015 - August 2016 (1 year and 1 month)Lucknow, India• Worked on characterization of infertility causing genes using rat models and developed a rat model with compromised spermatogenesis.• Developed a new protocol for double transfection of gene in-vitro which was hard to knockdown using a single transfection approach.• Performed and analyzed clinical semen samples using OMICS technologies.• Method development and validation of protocol for culturing primary cell lines used for infertility studies.• Designed and wrote a research study grant resulting in a 75000$ grant from Department of Biotechnology (DBT), India
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Education
- PhDSorbonne University and Institute Pasteur2020• Competitive scholarship from the Marie Skłodowska-Curie and Institut Pasteur to pursue PhD in the Genetics and Physiology of Audition lab • Investigated genetic, cellular and molecular links using technically challenging and unique mouse models involving hearing and balance disorders. • Supervised and supported graduate students. • Established complex experimental procedures in collaboration with colleagues. • Effectively collaborated with multiple national and international research labs.
- Bachelors and Masters of Technology in BiotechnologyAmity University2015• Conducted research on pH responsive hydrogels as a possible vehicle for drug delivery which lead to patent applications. • Hydrothermally synthesized and characterized carbon nanosheets and studied their antibacterial activity which lead to a first author publication in a young lab. • Synthesized a biocompatible nanomaterial for detection of nitric oxide leading to a publication. • Synthesized graphene quantum dots from sustainable sources. • Tested anti-cancer formulation incorporated in the hydrogel which was able to selectively cause apoptosis to cancer cells.
Certifications
- Animal ExperimentationInstitut Pasteur2016
Skill set (31)
Categories
- Other